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A microbiological safety cabinet (MSC) is a vital piece of equipment that helps to prevent exposure to biological hazards. All containment laboratory users and their supervisors should know how to use MSCs effectively, what the standards are for the different levels of containment, and how the facilities are managed. This course will help both users and managers understand their responsibilities in creating a safe working area and provide both theory and practical knowledge on the safe and effective use of tissue culture (Class II) cabinets.